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multiphor ii electrophoresis  (Danaher Inc)


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    Structured Review

    Danaher Inc multiphor ii electrophoresis
    Multiphor Ii Electrophoresis, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiphor/pmc11366540-58-9-20
    Average 86 stars, based on 1 article reviews
    multiphor ii electrophoresis - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Antiangiogenic Therapeutic mRNA Delivery Using Lung-Selective Polymeric Nanomedicine for Lung Cancer Treatment.
    Article Snippet: Therapeutic antibodies that block vascular endothelial growth factor (VEGF) show clinical benefits in treating nonsmall cell lung cancers (NSCLCs) by inhibiting tumor angiogenesis.. Nonetheless, the therapeutic effects of systemically administered anti-VEGF antibodies are often hindered in NSCLCs because of their limited distribution in the lungs and their adverse effects on normal tissues.. These challenges can be overcome by delivering therapeutic antibodies in their mRNA form to lung endothelial cells, a primary target of VEGF-mediated pulmonary angiogenesis, to suppress the NSCLCs.

    Article Title: The PGPR Bacillus aryabhattai promotes soybean growth via nutrient and chlorophyll maintenance and the production of butanoic acid
    Article Snippet: The IPG dry strips (4-10 NL IPG, 24cm, Genomine, Korea) were equilibrated for 12-16hrs with 7M urea, 2M thiourea containing 2% 3-CHAPS, 1% DTT, 1% pharmalyte and loaded with 200μg of the sample. .. Isoelectric focusing (IEF) was performed at 20°C using a Multiphor II electrophoresis system (Amersham Biosciences) following the manufacturer’s instructions. ..

    Article Title: The wheat TaF-box3, SCF ubiquitin ligase component, participates in the regulation of flowering time in transgenic Arabidopsis.
    Article Snippet: Histone methylation is actively involved in plant flowering time and is regulated by a myriad of genetic pathways that integrate endogenous and exogenous signals.. We identified an F-box gene from wheat (Triticum aestivum L.) and named it TaF-box3.. Transcript expression analysis showed that TaF-box3 expression was gradually induced during the floret development and anthesis stages (WS2.5–10).

    Article Title: Chemical Composition and Nutritional Value of Royal Jelly Samples Obtained from Honey Bee ( Apis mellifera ) Hives Fed on Oak and Rapeseed Pollen Patties
    Article Snippet: IPG dry strips (4–10 NL IPG, 24 cm, Genomine, Pohang, Republic of Korea) were equilibrated and loaded with the sample. .. Isoelectric focusing (IEF) was conducted using the Multiphor II electrophoresis unit and EPS 3500 XL power supply (Amersham Biosciences, Buckinghamshire, UK). ..

    Article Title: Proteome identification of common immunological proteins of two nematode parasites
    Article Snippet: .. Isoelectric focusing (IEF) was performed at 20oC using a Multiphor II electrophoresis unit with an EPS 3,500 XL power supply (Amersham Biosciences) following the manufacturer’s instructions. ..

    Article Title: Detection and pH-Thermal Characterization of Proteinases Exclusive of Honeybee Worker-Fate Larvae ( Apis mellifera L.).
    Article Snippet: .. Isoelectric focusing electrophoresis was performed at 15 ◦C on Multiphor II (Amersham Biosciences, Amersham, UK) with the following protocol: 500 V, 1 mA, 5 W, 1 Vh; 500 V, 1 mA, 5 W, 2500 Vh; 3500 V, 1 mA, 5 W, 10,000 Vh; 3500 V, 1 mA, 5 W, 33,250 Vh. ..

    Electrofocusing:

    Article Title: The PGPR Bacillus aryabhattai promotes soybean growth via nutrient and chlorophyll maintenance and the production of butanoic acid
    Article Snippet: The IPG dry strips (4-10 NL IPG, 24cm, Genomine, Korea) were equilibrated for 12-16hrs with 7M urea, 2M thiourea containing 2% 3-CHAPS, 1% DTT, 1% pharmalyte and loaded with 200μg of the sample. .. Isoelectric focusing (IEF) was performed at 20°C using a Multiphor II electrophoresis system (Amersham Biosciences) following the manufacturer’s instructions. ..

    Article Title: The wheat TaF-box3, SCF ubiquitin ligase component, participates in the regulation of flowering time in transgenic Arabidopsis.
    Article Snippet: Histone methylation is actively involved in plant flowering time and is regulated by a myriad of genetic pathways that integrate endogenous and exogenous signals.. We identified an F-box gene from wheat (Triticum aestivum L.) and named it TaF-box3.. Transcript expression analysis showed that TaF-box3 expression was gradually induced during the floret development and anthesis stages (WS2.5–10).

    Article Title: Chemical Composition and Nutritional Value of Royal Jelly Samples Obtained from Honey Bee ( Apis mellifera ) Hives Fed on Oak and Rapeseed Pollen Patties
    Article Snippet: IPG dry strips (4–10 NL IPG, 24 cm, Genomine, Pohang, Republic of Korea) were equilibrated and loaded with the sample. .. Isoelectric focusing (IEF) was conducted using the Multiphor II electrophoresis unit and EPS 3500 XL power supply (Amersham Biosciences, Buckinghamshire, UK). ..

    Article Title: Proteome identification of common immunological proteins of two nematode parasites
    Article Snippet: .. Isoelectric focusing (IEF) was performed at 20oC using a Multiphor II electrophoresis unit with an EPS 3,500 XL power supply (Amersham Biosciences) following the manufacturer’s instructions. ..

    Stripping Membranes:

    Article Title: Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis
    Article Snippet: .. Dithiothreitol (18.2 mM), PMSF (2 mM), and aprotinin (0.1 U/ml) were added to the rehydration solution just before the sample, and the mixture was centrifuged at 14,000 g for 15 min and applied via sample cup to a 7-cm isoelectric focusing strip (pre-equilibrated overnight in rehydration solution) for focusing at 20°C in a Multiphor II apparatus (Amersham Pharmacia) at 200 V for 1 min followed by 3500 V for 170 min. After storage at -75°C, strips were incubated for 15 min in 50 mM Tris, pH 8.8, plus 6 M urea, 30% glycerol, 2% SDS, trimmed to exclude ~7 mm from the anodic end, and submitted to 5% SDS–PAGE with an overlay of 0.5% agarose in 25 mM Tris, 192 mM glycine, and 0.1% SDS. ..

    Incubation:

    Article Title: Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis
    Article Snippet: .. Dithiothreitol (18.2 mM), PMSF (2 mM), and aprotinin (0.1 U/ml) were added to the rehydration solution just before the sample, and the mixture was centrifuged at 14,000 g for 15 min and applied via sample cup to a 7-cm isoelectric focusing strip (pre-equilibrated overnight in rehydration solution) for focusing at 20°C in a Multiphor II apparatus (Amersham Pharmacia) at 200 V for 1 min followed by 3500 V for 170 min. After storage at -75°C, strips were incubated for 15 min in 50 mM Tris, pH 8.8, plus 6 M urea, 30% glycerol, 2% SDS, trimmed to exclude ~7 mm from the anodic end, and submitted to 5% SDS–PAGE with an overlay of 0.5% agarose in 25 mM Tris, 192 mM glycine, and 0.1% SDS. ..



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    Image Search Results


    Specification of the Relevant Instrumental Techniques for LF Characterization

    Journal: Journal of Agricultural and Food Chemistry

    Article Title: The Multifaceted Roles of Bovine Lactoferrin: Molecular Structure, Isolation Methods, Analytical Characteristics, and Biological Properties

    doi: 10.1021/acs.jafc.3c06887

    Figure Lengend Snippet: Specification of the Relevant Instrumental Techniques for LF Characterization

    Article Snippet: , IEF , electromigration , Different pI values determined by IEF also might be a matter of the selected separation conditions, e.g. the type of carrier ampholyte (CA), pI marker, buffer composition, and separation time. The possibility of distinction of several LF fractions is based on their pI values. , Multiphor II apparatus (Amersham, Freiburg, Germany); precast gels Servalyt and Blank precotes (Serva, Germany); gel parameters, 126 × 125 × 0.3 mm; temperature, 5–8 °C; voltage, 2000 V; current, 6 mA; power, 12 W , ( , ) .

    Techniques: Isolation, Glycoproteomics, Staining, Mass Spectrometry, Concentration Assay, Spectrophotometry, Clinical Proteomics, Zeta Potential Analyzer, Marker, Spectroscopy, Software, Raman Spectroscopy, Functional Assay, Fluorescence, Sequencing, Single Particle, Microscopy, Chromatography, Solvent, Evaporation, Differential Scanning Calorimetry, Injection